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Figure 2. Mannan-elicited lymph node innate response requires Dectin-2-expressing, CD169+ sinus macrophages (A) WT, Clec4n/ and <t>Fcer1g/</t> mice were intradermally injected with saline or mannans. 24 h later dLNs were collected, their weights were measured, and RNA was extracted for gene expression analysis. Results are expressed as fold over contralateral, saline-injected LN. N = 3–5 mice per genotype.
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Figure 2. Mannan-elicited lymph node innate response requires Dectin-2-expressing, CD169+ sinus macrophages (A) WT, Clec4n/ and <t>Fcer1g/</t> mice were intradermally injected with saline or mannans. 24 h later dLNs were collected, their weights were measured, and RNA was extracted for gene expression analysis. Results are expressed as fold over contralateral, saline-injected LN. N = 3–5 mice per genotype.
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Figure 2. Mannan-elicited lymph node innate response requires Dectin-2-expressing, CD169+ sinus macrophages (A) WT, Clec4n/ and <t>Fcer1g/</t> mice were intradermally injected with saline or mannans. 24 h later dLNs were collected, their weights were measured, and RNA was extracted for gene expression analysis. Results are expressed as fold over contralateral, saline-injected LN. N = 3–5 mice per genotype.
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Figure 2. Mannan-elicited lymph node innate response requires Dectin-2-expressing, CD169+ sinus macrophages (A) WT, Clec4n/ and <t>Fcer1g/</t> mice were intradermally injected with saline or mannans. 24 h later dLNs were collected, their weights were measured, and RNA was extracted for gene expression analysis. Results are expressed as fold over contralateral, saline-injected LN. N = 3–5 mice per genotype.
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Figure 2. Mannan-elicited lymph node innate response requires Dectin-2-expressing, CD169+ sinus macrophages (A) WT, Clec4n/ and <t>Fcer1g/</t> mice were intradermally injected with saline or mannans. 24 h later dLNs were collected, their weights were measured, and RNA was extracted for gene expression analysis. Results are expressed as fold over contralateral, saline-injected LN. N = 3–5 mice per genotype.
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Image Search Results


Figure 2. Mannan-elicited lymph node innate response requires Dectin-2-expressing, CD169+ sinus macrophages (A) WT, Clec4n/ and Fcer1g/ mice were intradermally injected with saline or mannans. 24 h later dLNs were collected, their weights were measured, and RNA was extracted for gene expression analysis. Results are expressed as fold over contralateral, saline-injected LN. N = 3–5 mice per genotype.

Journal: Cell

Article Title: An adjuvant strategy enabled by modulation of the physical properties of microbial ligands expands antigen immunogenicity.

doi: 10.1016/j.cell.2022.01.009

Figure Lengend Snippet: Figure 2. Mannan-elicited lymph node innate response requires Dectin-2-expressing, CD169+ sinus macrophages (A) WT, Clec4n/ and Fcer1g/ mice were intradermally injected with saline or mannans. 24 h later dLNs were collected, their weights were measured, and RNA was extracted for gene expression analysis. Results are expressed as fold over contralateral, saline-injected LN. N = 3–5 mice per genotype.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER B6.Cg-Relbtm1Ukl/J, Relbfl/fl Jackson Laboratory Cat# 028719 B6.129S4-Ccr2tm1Ifc/J, Ccr2-/- Jackson Laboratory Cat# 004999 B6.129S(C)-Batf3tm1Kmm/J, Batf3-/- Jackson Laboratory Cat# 013755 B6.129-Card9tm1Xlin/J, Card9-/- Jackson Laboratory Cat# 028652 B6.129S6-Clec7atm1Gdb/J, Clec7a-/- Jackson Laboratory Cat# 012337 C57BL/6-Tg(TcraTcrb)1100Mjb/J, OT-I Jackson Laboratory Cat# 003831 B6.129P2-Fcer1gtm1Rav N12, Fcer1g-/- Taconic Cat# 583 Clec4n-/- Provided by Drs. Nora A. Barrett and Yoichiro Iwakura N/A B6;129-Siglec1 (CD169-DTR) Provided by Dr. F. Pucci from Riken Institute RBRC04395 B6.Cg-Tg(TcraTcrb)425Cbn/J, OT-II Jackson Laboratory Cat# 004194 VeroE6 cells ATCC Cat# CRL-1586 Specific-pathogen-free chicken (SPF) eggs Charles River Laboratories Cat# 10100329 Software and algorithms GraphPad Prism 8.0.2 GraphPad Prism - GraphPad RRID:SCR_002798 FlowJo software Tree star FlowJo v10.8 RRID:SCR_008520 ImageJ NIH ImageJ (nih.gov) RRID:SCR_003070 Amnis Ideas Software Luminex Corporation IDEAS 6.2 ll Article

Techniques: Expressing, Injection, Saline, Gene Expression